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Flow and Mass Cytometry
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Protocols and Useful Links

Key Resources

Current Protocols in Cytometry is available in hard copy in CN4158D.

  • Current Protocols in Cytometry is a "best practices" collection that distills and organizes the techniques of peer-reviewed protocols for flow and image cytometry available.

Current Protocols in Immunology is available in hard copy in CN4158D.

  • Current Protocols in Immunology is also a "best-practices" collection that provides comprehensive coverage of immunological methods.

Purdue Cytometry ListServ

A valuable forum for the discussion of many aspects of flow cytometry is the Purdue Cytometry Discussion List, which has an international following.

Persons may search the discussion archive. To subscribe to the discussion, visit this website. To post an email to the group, send an email to cytometry@lists.purdue.edu.

Links of interest

  • Cheeky Scientist Technical Programs Expert Cytometry Training
  • Fluorofinder Cytometry Reagent Resource
  • OMIP Panels
  • "The Immunology Link" CD Antigen Chart

Reference Links

  • Commonly Used Staining Protocols - Janis V. Giorgi Flow Cytometry Core Laboratory
  • BD Biosciences Flow Cytometry Training and E-Learning
  • ThermoFisher Flow Cytometry Resource Center
  • BioLegend Web Tools
  • DNA Analysis Protocols
  • Cell Cycle Basics
  • Fluorescence Tutorials -ThermoFisher
  • G-force calculator for your centrifuge - it's the g-force that's important, not the RPMs!!

Fluorescence Spectrum Viewers

  • Cytek Biosciences
  • Agilent
  • ThermoFisher

Antibody Cross-Reactivity Resources

  • BioLegend
  • NIH Nonhuman Primate Reagent Resource

Vendor Links

  • Cytek Biosciences (flow cytometers)
  • Agilent (flow cytometers)
  • Thermo Fisher (Attune flow cytometers, Bigfoot sorter (PROPEL Labs), antibodies, etc. - Invitrogen/Molecular Probes/eBioscience/Qdots)
  • Beckman Coulter (flow cytometers, antibodies, etc.)
  • Bio-Rad flow cytometry reagents
  • BioLegend (antibodies, reagents, etc.)
  • Jackson ImmunoResearch Laboratories (quality secondary antibodies)

Paid Software Links

  • FlowJo Flow Cytometry Software
  • FlowJo Tutorials (also check out Flowjo videos on YouTube)
  • De Novo FCS Express Flow Cytometry Software
  • Astrolabe Cytometry Platform (cloud based single-cell data analysis)

Free Software Links

  • MFI Flow Cytometry Software - Eric Martz
  • Flowing Software - Turku Center for Biotechnology
  • Bioconductor - an open source software (not specifically for flow cytometry) that uses the R statistical programming language and has some flow cytometry modules

Contact Us

Flow Cytometry

 Health Sciences Campus

GCC - M. Bert Storey Research Building

Jiji Xie, PhD

CN-3142

(706) 721-4157


Rebekah Tritz, PhD
Research Manager

CN-4158E

(706) 721-5468

rtritz@augusta.edu

 

Take Our Survey

The Georgia Cancer Center's Flow Cytometry Shared Resource values your feedback. Please, take the time to complete this survey to let us know how your experience was and if there are any opportunities for improvement. Thank you for working with us and for sharing your insights.

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